Called Anya by Orelov, was a Russian princess, the fourth and youngest daughter of Tsar Nicholas II and last survivor of the Romanov family.
During the slaughter of her family, the genetic memories of Chinese Assassin Shao Jun contained within Ezio Auditore's Precursor box bonded with Anastasia when she came in contact with both it and a shard of the Imperial Sceptre. While protected by the Russian Assassin Nikolai Orelov and tracked by both the Templars and the Red Army, Anastasia used her connection with Shao Jun to learn Assassin skills like freerunning, lockpicking and combat in order to survive. Eventually Anastasia left Russia for Germany, adopting the name Anna Anderson.