Against a lower-rated player, I prefer them on. Taking them off kind of lets the steam out of the position.
do you prefer playing with queens on or off

against lower rated opponents I prefer the queens are off the board. my opponent may make unpredictable moves and play a lot better. they may not be that good at coordinating the other pieces.
against higher rated I prefer to have my queen.

Some of it depends on the position. Generaly if I am ahead, I would probably want them off. One exception to that would be if I have the more active queen.
If I am behind, like I was in one game recently, I would prefer them on. Of course it depends if you can avoid not giving away too much material to keep them on. In a recent game, I managed to mate my opponent with a knight and queen combo, so it was definitely worth keeping that queen!
i know alot of it depends on the posistion, but usually what do you prefer