My guess is that 4.1 is mainly related to castling, to prevent the moving of both pieces at the same time.
Assuming someone had the habit of using both hands for normal moves, unless a handicap might require it, then my guess is that the arbiter would start with a warning and could work their way up the chain.
I imagine that rule is rarely invoked and due to the omission of 4.1 from section 5, it doesn't really matter as checkmate is on the board so the game ends.
Article 4.1 requires that each move must be made with one hand only.
Article 4.8 refers to opponent forfeiting the right to claim against 4.1 inter alia. Therefore some right to claim exist.
However, Article 5 repeatedly requires moves to comply with Articles 3 and 4.2 to 4.7 - comspicuously omitting 4.1.
So what is the penalty or remedy for 4.1 violations, if successfully claimed?