I have found about 200 comic books that feature chess on its cover or has a chess story. Here are some comic book characters that play chess. Are there any more?
Aladdin - Disney’s Aladdin plays chess. He appears on the Cover of Disney’s Aladdin #10 (1995) playing chess.
Alice – Alice (from Alice through the Looking Glass) appears in several comic books playing chess
Archie – Archie Andrews appears on a few comic book covers and in a few comic strips playing chess.
Batman – Batman appears on one comic book cover (Batman #24, June-July 1944) playing the Joker in chess. He has a giant chess set in the bat cave.
Betty – Betty and Veronica are on the cover of an Archie comic book (1950) playing chess
Bizzaro – plays chess. Beat Lex Luthor in a game of chess.
Black Panther – The Black Panther (T’Challa) plays chess with Dr. Doom.
Brainiac 5 – plays chess against Marshall Lorca of the Khunds
Captain Comet – Fictional DC Comics superhero who plays chess. The Guardians of the Universe send Captain Comet (Adam Blake) on a mission to win a chess tournament. He holds his own with Xtnal from Venus. He defeats Pyg-Gar, a grandmaster from Pluto. He is able to see 200 moves in advance. (Strange Adventures, ‘The Cosmic Chessboard,’ 1953)
Casper – Casper the Friendly ghost appears on a cover (Casper #117, 1968) playing chess.
Cerebus the Aardvark – Plays chess with his friend Po.
Chequerians – Masters of the checks and balances of the universe. They play chess with entire planets as chess pieces.
Chessmen, The – Team name of personal assassin of Millionaire industrialist Obadiah Shane, who is trying to eliminated competitor Tony Stark (Ironman). Shane was brought up with a great respect for chess, so he patterned his actions associated with the game. Members include the King, Queen, Bishop, Knight, and Rook.
Chess Set, The – Super-powered criminal individuals named Brass Bishop, Dark Tower, King Coal, Killer Queen and Over-Knight. They first appeared in Marvel’s Alpha Flight #121 (1983).
Despero – Villain who first appeared in Justice League of America #1 (1960). He likes to play chess for the fates of his enemies. He defeats the Flash in a game similar to chess by cheating. He also plays the Martian Manhunter in a game of chess.
Donald Duck – appears on a comic cover playing chess.
Dormammu – a mystical entity who plays chess with Odin.
Dr. Doom – Dr. Victor von Doom likes to play chess. He plays chess against the Prime Mover in which real people are pitted against robots constructed by Doom. He also plays chess with the Black Panther (T’Challa) and the Sub-Mariner.
Dr. Fate – Doctor Fate (Kent Nelson) takes part in a chess game in which humans take the roles of chess pieces in a huge board. (‘King of Crime,’ More Fun Comics #76, 1942)
Dr. Mid-Nite – forced to play chess with Mr. Terrific. The loser would be electrocuted.
Dr. Wrath – All who oppose this chess master die (‘King of the Chess Killers,’ The Blue Beetle #11, 1942).
Flash – plays Despero in a game of chess and loses because Despero cheated.
Hulk – makes a blunder in chess and the game is stalemated against Galactus.
Galactus – Devours worlds. Plays chess with the Avengers after being challenged by Storm. The game ends in a stalemated due to a blunder by Hulk.
Grandmaster, The – one of the ageless Elders of the Universe who has mastered most civilizations’ games of skill and chance, including chess.
Green Arrrow – plays chess (‘The Human Chess game,’ Adventure Comics, 1955)
Joker – plays Batman in chess.
Kang – Kang (Nathaniel Richards) plays chess with the Grandmaster for the life of Ravonna.
Karno the Chessman – a villain who plays chess.
Kingpin – Kingpin (Wilson Fink), a villain, plays chess with The Foreigner
Lex Luthor – plays chess. Got beat by Bizarro.
Manhunter from Mars – plays cosmic-level chess with Despero using Justice League of America figures as chess pieces.
Mister Mxyztptlk – impish villain from the 5th dimension. Learned how to play chess from Superman’s advice.
Mr. Terrific - forced to play chess with Dr. Mid-Nite. The loser would be electrocuted.
Odin – plays cosmic chess with Dormammu
Prime Mover – Robot employed by Doctor doom to play chess.
Prince Valiant - plays chess
Rathcone – Nazi operative operating in the United States in the 1940s who played chess. He plotted murders using a chess board with chess pieces that resembled real people.
Richie Rich – played chess with his expensive chess set
Sabrina – plays chess
Seaguy – plays chess with Death in New Venice
Sub-Mariner plays chess with Dr. Drrom.
Superman – plays chess on a few comics covers.
Thor – Appeared on some comic covers playing chess.
Veronica - Betty and Veronica are on the cover of an Archie comic book (1950) playing chess
Wizard – The Wizard (Bentley Wittman) was a child prodigy and chess champion who became a supervillain.
Yugi Muto – plays chess against Mokuba in a Capsule Monster Chess game and wins.